Cloned (Comment) | Organism |
---|---|
transient expression of mature enzyme and pro-enzyme in COS-1 cells, in vitro transcritption and translation | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
endoplasmic reticulum | the recombinant precusor in COS-1 cells resides in the ER, not mature wild-type | Homo sapiens | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | mannose-rich, complex glycosylated mature enzyme, dependent on ooptimal folding of the processed proform in presence of the pro-peptide | Homo sapiens |
proteolytic modification | enzyme is sythesized as a transport-competent precursor, i.e. pro-LPH, intracellular cleavage process in the trans-Golgi network between Arg734 and Leu 735 to yield the mature enzyme, i.e. LPHbetainitial, the propeptide of the zymogen, i.e. LPHalpha, modulates as a chaperone the folding of the mature enzyme after it has been cleaved off, mechanism, overview | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant mature enzyme and pro-enzyme from COS-1 cells | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phloretin + H2O = phloretate + phloroglucinol | also hydrolyses other C-acetylated phenols related to phloretin | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | - |
Homo sapiens | beta-D-galactose + beta-D-glucose | - |
? | |
additional information | the enzyme interacts with ER chaperones, immunglobulin binding protein, and calnexin at non-permissive temperatures in the ER | Homo sapiens | ? | - |
? | |
phloretin + H2O | - |
Homo sapiens | phloretate + phloroglucinol | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the mature enzyme shows a temperature-sensitive conformation, interaction with ER chaperones, immunglobulin binding protein, and calnexin, enzyme structure, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
lactase-phlorizin hydrolase | - |
Homo sapiens |
LPH | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the mature enzyme shows a temperature-sensitive conformation | Homo sapiens |